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Nasri, 30, had appealed to UEFA for a therapeutic use exemption (TUE) following a multi-vitamin intravenous booster he received at a Los Angeles clinic in 2016.
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Former Manchester City midfielder and France international Samir Nasri was handed a six-month doping ban "for using a prohibited method" by European football governing body UEFA on Tuesday.
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That was rejected last March, as was an appeal in December to the Court of Arbitration for Sport.
The method is on the World Anti-doping Agency's prohibited list.
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